Osteocalcin/fibronectin-functionalized collagen matrices for bone tissue engineering

Title
Osteocalcin/fibronectin-functionalized collagen matrices for bone tissue engineering
Authors
장준혁
Keywords
rhOCN/FNIII9-10, MC3T3-E1, collagen matrices, cell adhesion, osteogenic differentiation, bone tissue engineering
Issue Date
2015-06
Publisher
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Series/Report no.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A ; Vol103 no.6 Startpage 2133 Endpage 2140
Abstract
Collagen is the most abundant protein found in the extracellular matrix and is widely used to build scaffolds for biomedical applications which are the result of its biocompatibility and biodegradability. In the present study, we constructed a rhOCN/FNIII9‐10 fusion protein and rhOCN/FNIII9‐10‐functionalized collagen matrices and investigated the potential value for bone tissue engineering. In vitro studies carried out with preosteoblastic MC3T3‐E1 cells showed that rhOCN/FNIII9‐10 fusion protein promoted cell adhesion and the mRNA levels of osteogenic markers including osteocalcin, runt‐related transcription factor 2, alkaline phosphatase (ALP), and collagen type I. In addition, rhOCN/FNIII9‐10‐functionalized collagen matrices showed significant induction of the ALP activity more than rhFNIII9‐10‐functionalized collagen matrices or collagen matrices alone. These results suggested that rhOCN/FNIII9‐10‐functionalized collagen matrices have potential for bone tissue engineering.
URI
https://onlinelibrary.wiley.com/doi/abs/10.1002/jbm.a.35351
http://dspace.inha.ac.kr/handle/10505/55173
ISSN
1549-3296
Appears in Collections:
Medical School/College of Medicine (의학전문대학원/의과대학) > Medical Science (의학) > Journal Papers, Reports(의학 논문, 보고서)

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