Pikromycin Production Stimulation through Antibiotic Down-regulatory Gene Disruption in Streptomyces venezuelae

Title
Pikromycin Production Stimulation through Antibiotic Down-regulatory Gene Disruption in Streptomyces venezuelae
Authors
김응수
Keywords
wblA, Pikromycin, Streptomyces venezuelae
Issue Date
2014-12
Publisher
Biotechnology and Bioprocess Engineering
Series/Report no.
Biotechnology and Bioprocess Engineering; 19권 6호 pp 973~977
Abstract
The wblA gene, which encodes a whiB-like putative transcription factor, has been widely reported as an antibiotic biosynthesis down-regulator in Streptomyces coelicolor. The wblA ortholog from Streptomyces venezuelae was identified by sequence alignment with wblA from S. coelicolor. To determine the biological significance of wblA from S. venezuelae, chromosomal disruption of the wblAsve gene was performed by homologous recombination using the pKC1139 vector. The phenotypic difference between S. venezuelae and S. venezuelae∆wblAsve was shown, and pikromycin production was quantified by HPLC analysis. Production of pikromycin by S. venezuelae∆wblAsve was approximately 3.5-fold higher compared to that by S. venezuelae. To further show that the wblAsve gene was implicated in pikromycin production, wblAsve was cloned into Streptomyces integrative expression vector, which was independently introduced into both S. venezuelae and S. venezue∆laewblAsve by conjugation. HPLC analysis of complementation and overexpression strains showed an approximately 2.5-fold reduction in pikromycin production by the wblAsve overexpression mutant.
URI
http://dspace.inha.ac.kr/handle/10505/39168
ISSN
1226-8372
Appears in Collections:
College of Engineering(공과대학) > Biotechnology (생명공학) > Local Access Journal, Report (생명공학 논문, 보고서)

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