Mesenchymal stem cell lines isolated by different isolation methods show variations in the regulation of graft-versus-host disease

Title
Mesenchymal stem cell lines isolated by different isolation methods show variations in the regulation of graft-versus-host disease
Authors
김우철; 전명신; 송순욱
Keywords
Mesenchymal stem cells, graft-versus-host disease, T-cell, IFN-γ, Efficacy
Issue Date
2013
Publisher
IMMUNE NETWORK
Series/Report no.
IMMUNE NETWORK ; 제13권 제4호 pp 133~140
Abstract
Since the discovery of the immunomodulation property of mesenchymal stem cells (MSCs) about a decade ago, it has been extensively investigated whether MSCs can be used for the treatment of immune-related diseases, such as graftversus-host disease (GvHD). However, how to evaluate the efficacy of human MSCs for the clinical trial is still unclear. We used an MHC-mismatched model of GvHD (B6 into BALB/c). Surprisingly, the administration of the human MSCs (hMSCs) could reduce the GvHD-related mortality of the mouse recipients and xenogeneically inhibit mouse T-cell proliferation and IFN-γ production in vitro. We recently established a new protocol for the isolation of a homogeneous population of MSCs called subfractionation culturing methods (SCM), and established a library of clonal MSC lines. Therefore, we also investigated whether MSCs isolated by the conventional gradient centrifugation method (GCM) and SCM show different efficacy in vivo. Intriguingly, clonal hMSCs (hcMSCs) isolated by SCM showed better efficacy than hMSCs isolated by GCM. Based on these results, the MHC-mismatched model of GvHD may be useful for evaluating the efficacy of human MSCs before the clinical trial. The results of this study suggest that different MSC lines may show different efficacy in vivo and in vitro.
URI
http://dx.doi.org/10.4110/in.2013.13.4.133
http://dspace.inha.ac.kr/handle/10505/33841
ISSN
1598-25629
Appears in Collections:
Medical School/College of Medicine (의학전문대학원/의과대학) > Medical Science (의학) > Local Access Journal Papers, Reports(의학 논문, 보고서)

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse