Valproic acid decreases the proliferation of telencephalic cells in zebrafish larvae

Title
Valproic acid decreases the proliferation of telencephalic cells in zebrafish larvae
Authors
이창중
Keywords
Danio rerio Valproic acid Cell proliferation wnt signaling
Issue Date
2013
Publisher
NEUROTOXICOLOGY AND TERATOLOGY
Series/Report no.
NEUROTOXICOLOGY AND TERATOLOGY ; Vol39 Startpage 91 Endpage 99
Abstract
Valproic acid (VPA) is used as an antiepileptic drug (AED) or mood stabilizer. Recent studies have shown that exposure to VPA during embryonic development alters neural progenitor cell (NPC) proliferation, and can also lead to behavioral impairment in adult animals. The main goal of this investigation was to evaluate the effects of treatment with 2 mM VPA for 3 h on cell proliferation in the telencephalic area of zebrafish larvae of 5 days postfertilization (dpf) using immunohistochemistry (IHC). It was also determined whether VPA exposure affects the learning ability and anxiety levels of zebrafish during adulthood using bottom-dwelling behavior and passive avoidance tests. Result of the study demonstrated that VPA exposure during development transiently decreased neuronal cell proliferation without inducing apoptosis. Additionally, quantitative real-time PCR (qRT-PCR) data indicated that mRNA expression levels of wnt signaling pathway-related factors such as β-catenin, lef1, and gsk3β were altered in the zebrafish treated with VPA. Interestingly, these effects were reversed over time after VPA treatment had ceased. Alterations of passive avoidance learning and bottom dwelling behaviorwere not observed during adulthood after developmental VPA exposure. These results may be due to the restoration of cell proliferation during the recovery period after VPA treatment.
URI
http://dx.doi.org/10.1016/j.ntt.2013.07.004
http://dspace.inha.ac.kr/handle/10505/33252
ISSN
0892-0362
Appears in Collections:
College of Natural Science(자연과학대학) > Biological Sciences (생명과학) > Journal Papers, Reports(생명과학 논문, 보고서)
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