Enhanced Phase II Detoxification Contributes to Beneficial Effects of Dietary Restriction as Revealed by Multi-platform Metabolomics Studies

Title
Enhanced Phase II Detoxification Contributes to Beneficial Effects of Dietary Restriction as Revealed by Multi-platform Metabolomics Studies
Authors
민경진
Issue Date
2013
Publisher
MOLECULAR & CELLULAR PROTEOMICS
Series/Report no.
MOLECULAR & CELLULAR PROTEOMICS ; Vol12 no.3 Startpage 575 Endpage 586
Abstract
Dietary restriction (DR) has many beneficial effects, but the detailed metabolic mechanism remains largely unresolved. As diet is essentially related to metabolism, we investigated the metabolite profiles of urines from control and DR animals using NMR and LC/MS metabolomic approaches. Multivariate analysis presented distinctive metabolic profiles and marker signals from glucuronide and glycine conjugation pathways in the DR group. Broad profiling of the urine phase II metabolites with neutral loss scanning showed that levels of glucuronide and glycine conjugation metabolites were generally higher in the DR group. The up-regulation of phase II detoxification in the DR group was confirmed by mRNA and protein expression levels of uridinediphospho-glucuronosyltransferase and glycine-N-acyltransferase in actual liver tissues. Histopathology and serum biochemistry showed that DR was correlated with the beneficial effects of low levels of serum alanine transaminase and glycogen granules in liver. In addition, the Nuclear factor (erythroid-derived 2)-like 2 signaling pathway was shown to be up-regulated, providing a mechanistic clue regarding the enhanced phase II detoxification in liver tissue. Taken together, our metabolomic and biochemical studies provide a possible metabolic perspective for understanding the complex mechanism underlying the beneficial effects of DR.
URI
http://dspace.inha.ac.kr/handle/10505/33224
ISSN
1535-9476
Appears in Collections:
College of Natural Science(자연과학대학) > Biological Sciences (생명과학) > Local Access Journal Papers, Reports(생명과학 논문, 보고서)

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse