Diversity and selective pressures of anticoagulants in three medicinal leeches (Hirudinida: Hirudinidae, Macrobdellidae)

Title
Diversity and selective pressures of anticoagulants in three medicinal leeches (Hirudinida: Hirudinidae, Macrobdellidae)
Authors
민기식
Keywords
Anticoagulant, evolution, FEL, Hirudinida, medicinal leeches, PARRIS, REL, selection pressures, SLAC.
Issue Date
2013
Publisher
ECOLOGY AND EVOLUTION
Series/Report no.
ECOLOGY AND EVOLUTION ; Vol3 no.4 Startpage 918 Endpage 933
Abstract
Although medicinal leeches have long been used as treatment for various ailments because of their potent anticoagulation factors, neither the full diversity of salivary components that inhibit coagulation, nor the evolutionary selection acting on them has been thoroughly investigated. Here, we constructed expressed sequence tag libraries from salivary glands of two species of medicinal hirudinoid leeches, Hirudo verbana and Aliolimnatis fenestrata, and identified anticoagulant-orthologs through BLASTx searches. The data set then was augmented by the addition of a previously constructed EST library from the macrobdelloid leech Macrobdella decora. The identified orthologs then were compared and contrasted with well-characterized anticoagulants from a variety of leeches with different feeding habits, including non-sanguivorous species. Moreover, four different statistical methods for predicting signatures of positive and negative evolutionary pressures were used for 10 rounds each to assess the level and type of selection acting on the molecules as a whole and on specific sites. In total, sequences showing putative BLASTx-orthology with five and three anticoagulant-families were recovered in the A. fenestrata and H. verbana EST libraries respectively. Selection pressure analyses predicted high levels of purifying selection across the anticoagulant diversity, although a few isolated sites showed signatures of positive selection. This study represents a first attempt at mapping the anticoagulant repertoires in a comparative fashion across several leech families.
URI
http://dspace.inha.ac.kr/handle/10505/33223
ISSN
2045-7758
Appears in Collections:
College of Natural Science(자연과학대학) > Biological Sciences (생명과학) > Local Access Journal Papers, Reports(생명과학 논문, 보고서)

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