Polystyrene bound stationary phase of excellent separation efficiency based on partially sub-2 μm silica monolith particles

Title
Polystyrene bound stationary phase of excellent separation efficiency based on partially sub-2 μm silica monolith particles
Authors
정원조
Keywords
Partially sub-2 m silica monolith particles Initiator attachment RAFT polymerization Uniform and thin polystyrene film Exceptional high separation efficiency
Issue Date
2013
Publisher
JOURNAL OF CHROMATOGRAPHY A
Series/Report no.
JOURNAL OF CHROMATOGRAPHY A ; Vol1303 Startpage 9 Endpage 17
Abstract
Partially sub-2 m porous silica monolith particles have been synthesized by a renovated procedure and modified to polystyrene coated silica particles with excellent separation efficiency when used as chromatographic media. In the procedure of preparing silica monolith particles in this study, subtle control of formulation of the reaction mixture and multi-step heating followed by calcination, without any washing and sieving process, enabled formation of silica particles characterized by proper particle and pore size distribution for high separation efficiency. 3-Chloropropyl trimethoxysilane was used as the halogen terminal spacer to combine the initiator to silica particles. Uniform and thin coating of polystyrene layer on initiator attached silica particles was formed via reversible addition-fragmentation chain transfer (RAFT) polymerization. Micro-columns (1.0 mm ID and 300 mm length) were packed with the resultant phase and their chromatographic performance was elucidated by HPLC. A mobile phase of 60/40 (v/v) acetonitrile/water containing 0.1% TFA and a flow rate of 15 L/min were found to be the optimized conditions leading to number of theoretical plates close to 50,000 (165,000 m?1). This is the very first study to get such highly efficient HPLC columns using a silica monolith particulate stationary phase.
URI
http://dx.doi.org/10.1016/j.chroma.2013.06.016
http://dspace.inha.ac.kr/handle/10505/33169
ISSN
0021-9673
Appears in Collections:
College of Natural Science(자연과학대학) > Chemistry (화학) > Journal Papers, Reports(화학 논문, 보고서)
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