High throughput detection and selective enrichment of histidine-tagged enzymes with Ni-doped magnetic mesoporous silica

Title
High throughput detection and selective enrichment of histidine-tagged enzymes with Ni-doped magnetic mesoporous silica
Authors
박상언
Issue Date
2013
Publisher
JOURNAL OF MATERIALS CHEMISTRY B
Series/Report no.
JOURNAL OF MATERIALS CHEMISTRY B ; Vol1 no.5 Startpage 610 Endpage 616
Abstract
This work reports a simple and facile method to prepare novel magnetic mesoporous silica (MMS) materials with high magnetic strength for the convenient and high throughput detection of histidine-tagged enzymes with Ni-doped surfaces. These materials are designed by the incorporation of high-abundance and homogeneously dispersed iron nanoparticles within the mesopores by thermal hydrogen reduction after the incorporation of ferrous ions and demonstrated the selective enrichment and high-throughput recognition of His-tagged enzymes with multi-point anchoring by selective conjugation between the His-tag and Ni ions. Selective His-tagged enzyme enrichment efficiency was compared with nickel-based MMS materials, such as Ni2+-MMS and Ni-MMS, and nickel ion doped silica-coated magnetic nanoparticles (Ni2+-MNPs). The efficiency was calculated to be 100 1.93%, 70.94 1.95%, and 37.03 5.93% for Ni2+-MMS, Ni-MMS, and Ni2+-MNPs, respectively. This method enables a highthroughput and advanced systematic approach for the separation and immobilization of proteins which cover a broad spectrum of polyhistidine-tagged proteins.
URI
http://dspace.inha.ac.kr/handle/10505/33157
ISSN
2050-750X
Appears in Collections:
College of Natural Science(자연과학대학) > Chemistry (화학) > Journal Papers, Reports(화학 논문, 보고서)
Files in This Item:
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