Memory-Enhancing Effects of Silk Fibroin-Derived Peptides in Scopolamine-Treated Mice

Title
Memory-Enhancing Effects of Silk Fibroin-Derived Peptides in Scopolamine-Treated Mice
Authors
이우주
Keywords
Peptide, memory, fibroin, silk, scopolamine
Issue Date
2013
Publisher
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Series/Report no.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY ; Vol23 no.12 Startpage 1779 Endpage 1784
Abstract
Although enzyme-hydrolyzed silk fibroin has been reported to enhance cognitive function before, it has been still unknown which peptides can improve memory. Here we report that amino acid sequences of three novel peptides were identified from fibroin hydrolysate. Fibroin hydrolysate was obtained by hydrolysis with protease after partial hydrolysis with 5M CaCl2. Synthesized peptides derived from these sequences improved scopolamine-induced memory impairments in mice. We confirmed this hydrolysate had effects that improved learning and memory abilities by performing the Rey-Kim test. From this hydrolysate of silk fibroin, amino acid sequences of eight peptides were identified by LC-MS/MS. Three peptides (GAGAGTGSSGFGPY, GAGAGSGAGSGAGAGSGAGAGY, and SGAGSGAGAGSGAGAGSGA) were synthesized to investigate whether they could improve memory. Passive avoidance test and Morris water maze test were performed, and all peptides showed memory-enhancing abilities on scopolamine-induced memory impairments in mice. In this study, we identified three novel peptides that could improve memory, and that silk fibroin hydrolysate was a mixture of various active peptides that could enhance memory.
URI
http://dx.doi.org/10.4014/jmb.1308.08059
http://dspace.inha.ac.kr/handle/10505/33079
ISSN
1017-7825
Appears in Collections:
College of Natural Science(자연과학대학) > Statistics (통계학) > Local Access Journal Papers, Reports(통계학 논문, 보고서)

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