Antibiotic resistance of Shewanella putrefaciens isolated from shellfish collected from the West Sea in Korea

Title
Antibiotic resistance of Shewanella putrefaciens isolated from shellfish collected from the West Sea in Korea
Authors
소재성
Issue Date
2013
Publisher
MARINE POLLUTION BULLETIN
Series/Report no.
MARINE POLLUTION BULLETIN; vol.76 no.1-2 startpage 85 endpage 88
Abstract
In this study, we isolated and characterized Shewanella putrefaciens from shellfish harvested from the West Sea in Korea. For the initial isolation of S. putrefaciens, LB agar plates supplemented with ferrous sulfate and sodium thiosulfate were inoculated with shellfish homogenates, incubated for 24 h, and then black colonies were selected. Gram-negative and catalase-positive colonies were subsequently confirmed by PCR assays and API 20E kit test system. The Shewanella-specific 16S rRNA and gyrB genes were used to design S. putrefaciens-specific PCR primers. From 6 species of shellfish tested, 24 S. putrefaciens strains were isolated. These 24 isolates had the following profiles of resistance against 16 antibiotics: all the isolates were resistant to cephalothin and vancomycin and 95.8% were resistant to ampicillin. Here, we report the isolation of S. putrefaciens from shellfish and our results point to a new possible route for exposing healthy individuals to S. putrefaciens.
URI
http://dx.doi.org/10.1016/j.marpolbul.2013.09.025
http://dspace.inha.ac.kr/handle/10505/31997
ISSN
0025-326X
Appears in Collections:
College of Engineering(공과대학) > Biotechnology (생명공학) > Journal Papers, Reports(생명공학 논문, 보고서)
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