Process Characterization of hCTLA4Ig Production in Transgenic Rice Cell Cultures Using a 3-L Bioreactor

Title
Process Characterization of hCTLA4Ig Production in Transgenic Rice Cell Cultures Using a 3-L Bioreactor
Authors
김동일
Keywords
Transgenic rice cell culture . Process characterization . Agitationandaeration rates . Fed-batch cultivation
Issue Date
2013
Publisher
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Series/Report no.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY; vol.171 startpage 1276 endpage 1288
Abstract
Most of the technical know-how and experience of bioreactor engineering is applicable to plant cell cultures. In this study, transgenic rice cell cultures using RAmy3D promoter were used for the production of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig). In process aspect, the rice cells during production phase are strongly influenced by hydrodynamic stresses, such as shear stress and bubble burst. Therefore, the effects of agitation and aeration rates on cell growth and hCTLA4Ig production were investigated in a 3-L multi-bioreactor. By increasing over 240 rpm, the detrimental effects on cell growth and hCTLA4Ig production were observed. At an aeration rate of 0.3 vvm, relative cell viability sharply decreased 2 days earlier than those of lower aeration rates. In addition, it was confirmed that the specific yields and the specific productivity at 0.3 vvm were superior to those values at 0.05 vvm. Overall, higher aeration rate showed the improved hCTLA4Ig production in combination experiment. High aeration rates in general, however, have an undesired effect as excessive aeration was found to negatively affect the quality of hCTLA4Ig. Consequently, the hydrodynamic conditions must be tightly regulated during bioreactor operation in order to enhance hCTLA4Ig productivity and quality in transgenic rice cell cultures.
URI
http://dspace.inha.ac.kr/handle/10505/31995
ISSN
0273-2289
Appears in Collections:
College of Engineering(공과대학) > Biotechnology (생명공학) > Journal Papers, Reports(생명공학 논문, 보고서)
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