Bioreactor Engineering Using Disposable Technology for Enhanced Production of hCTLA4Ig in Transgenic Rice Cell Cultures

Title
Bioreactor Engineering Using Disposable Technology for Enhanced Production of hCTLA4Ig in Transgenic Rice Cell Cultures
Authors
김동일
Keywords
transgenic rice cell cultures; plant-specific bioprocess; disposable bioreactor; bioreactor engineering; mass and oxygen transfer
Issue Date
2013
Publisher
BIOTECHNOLOGY AND BIOENGINEERING
Series/Report no.
BIOTECHNOLOGY AND BIOENGINEERING; vol.110 no.9 startpage 2412 endpage 2424
Abstract
Two kinds of disposable bioreactors, air-lift disposable bioreactors (ADB) and wave disposable bioreactors (WDB) were compared with stirred-tank reactors (5-L STR). These bioreactors were successfully applied to transgenic rice cell cultures for the production of recombinant human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig). In both systems, a fed-batch culture method was used to produce hCTLA4Ig efficiently by feeding concentrated amino acids and production levels were enhanced when dissolved oxygen (DO) level was regulated at 30% using pure oxygen sparging. Agitation and aeration rate during cultivation in ADB and WDB were determined by the same mixing time. The results in both disposable bioreactors showed similar values in maximum cell density (11.9 gDCW/L and 12.6 gDCW/L), doubling time (4.8- and 5.0-day), and maximum hCTLA4Ig concentration (43.7 and 43.3 mg/L). Relatively higher cell viability was sustained in the ADB whereas hCTLA4Ig productivity was 1.2-fold higher than that in WDB. The productivity was improved by increasing aeration rate (0.2 vvm). Overall, our experiments demonstrate pneumatically driven disposable bioreactors are applicable for the production of recombinant proteins in plant cell cultures. These results will be useful for development and scale-up studies of disposable bioreactor systems for transgenic plant cell cultures.
URI
http://dspace.inha.ac.kr/handle/10505/31994
ISSN
0006-3592
Appears in Collections:
College of Engineering(공과대학) > Biotechnology (생명공학) > Local Access Journal, Report (생명공학 논문, 보고서)

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