Refolding of Laccase in Dilution Additive Mode with Copper-Based Ionic Liquid

Title
Refolding of Laccase in Dilution Additive Mode with Copper-Based Ionic Liquid
Authors
구윤모
Keywords
Copper-containing protein . Dilution refolding . Ionic liquid . Laccase . Metalloenzyme . Protein refolding
Issue Date
2013
Publisher
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Series/Report no.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY; vol.171 no.5 startpage 1289 endpage 1298
Abstract
Ionic liquids (ILs) are molten salts which do not crystallize at room temperature. Tunable physicochemical properties of ILs including hydrophobicity and polarity facilitate their applications in many biological processes. In this study, a copper-based IL was employed in order to enhance the refolding efficiency of laccase from Trametes versicolor which requires copper as a cofactor. When 1-ethyl-3-methylimidazolium trichlorocuprate ([EMIM][CuCl3]) was added to refolding buffer instead of urea, the laccase refolding yield was improved more than 2.7 times compared to the conventional refolding buffer which contains urea. When the refolding of laccase was carried out at different temperatures (4, 25, and 37 °C), the highest refolding yield was obtained at 25 °C. At low temperature, two conflicting effects, i.e., suppression of the aggregate formation and decrease of folding rate, influence the protein refolding. In contrast, a copper-based IL did not enhance the refolding of lysozyme, a non-copper-containing protein. From these results, we can conclude that this copper-based IL, [EMIM][CuCl3], was exclusively effective on the refolding process of a copper-containing protein.
URI
http://dspace.inha.ac.kr/handle/10505/31989
ISSN
0273-2289
Appears in Collections:
College of Engineering(공과대학) > Biotechnology (생명공학) > Journal Papers, Reports(생명공학 논문, 보고서)
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