Rapid detection and quantification of bacteria using an integrated micro/nanofluidic device

Title
Rapid detection and quantification of bacteria using an integrated micro/nanofluidic device
Authors
김선민; 전태준
Keywords
Bacteria Escherichia coli (E. coli) Detection Preconcentration Micromixer
Issue Date
2013
Publisher
SENSORS AND ACTUATORS B-CHEMICAL
Series/Report no.
SENSORS AND ACTUATORS B-CHEMICAL; vol.178 startpage 683 endpage 688
Abstract
In this study, we present an integrated micro/nanofluidic device that integrates a micromixer and a preconcentrator for the rapid detection of bacteria. The micromixer based on the concept of unbalanced splits and cross-collisions of fluid streams is used for mixing bacterial cells with tagging molecules. The preconcentrator consists of two microchannels (main and sub) that are connected by the nanochannels, which can be easily fabricated by electric breakdown of a ∼25 m-thick polydimethylsiloxane (PDMS) membrane using high electric shock. Escherichia coli (E. coli) sample is tagged with fluorescent dye and continuously preconcentrated at the target position by applying the electric field through the junction of micro- and nanochannels. The concentration of bacterial sample can be quantified by measuring the fluorescence intensity at the preconcentrated region.
URI
http://dx.doi.org/10.1016/j.snb.2013.01.017
http://dspace.inha.ac.kr/handle/10505/31610
ISSN
0925-4005
Appears in Collections:
College of Engineering(공과대학) > Mechanical Engineering(기계공학) > Journal Papers, Reports(기계공학 논문, 보고서)
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