Improvement in sensory function via granulocyte-macrophage colony-stimulating factor in rat spinal cord injury models Laboratory investigation

Title
Improvement in sensory function via granulocyte-macrophage colony-stimulating factor in rat spinal cord injury models Laboratory investigation
Authors
박현선
Keywords
granulocyte-macrophage colony-stimulating factor ? rat ??spinal cord injury ? sensory recovery ? calcitonin gene-related peptide ? trauma
Issue Date
2013
Publisher
JOURNAL OF NEUROSURGERY-SPINE
Series/Report no.
JOURNAL OF NEUROSURGERY-SPINE ; Vol.18 no.1 Startpage 69 Endpage 75
Abstract
Object. The aim in this study was to determine whether granulocyte-macrophage colony-stimulating factor (GM-CSF) leads to sensory improvement in rat spinal cord injury (SCI) models. Methods. Thirty male Sprague-Dawley rats were included in this study: 10 in the sham group (laminectomy alone without SCI), 10 in the SCI group (SCI treated with phosphate-buffered saline), and 10 in the GM-CSF treatment group (SCI treated with GM-CSF). A locomotor function test and pain sensitivity test were conducted weekly for 9 weeks after SCI or sham injury. Spinal tissue samples from all rats were immunohistochemically examined for the expression of calcitonin gene-related peptide (CGRP) and abnormal sprouting at Week 9 post-SCI. Results. Granulocyte-macrophage colony-stimulating factor treatment improves functional recovery after SCI. In the tactile withdrawal threshold and frequency of the hindlimb paw, the GM-CSF group always responded with a statistically significant lower threshold than the SCI group 9 weeks after SCI (p < 0.05). The response of the forelimb and hindlimb paws to cold in the GM-CSF group always reflected a statistically significant lower threshold than in the SCI group 9 weeks after injury (p < 0.05). Decreased CGRP expression, observed by density and distribution area, was noted in the GM-CSF group (optical density 113.5 ± 20.4) compared with the SCI group (optical density 143.1 ± 18.7; p < 0.05). Conclusions. Treatment with GM-CSF results in functional recovery, improving tactile and cold sense recovery in a rat SCI model. Granulocyte-macrophage colony-stimulating factor also minimizes abnormal sprouting of sensory nerves after SCI.
URI
http://thejns.org/doi/abs/10.3171/2012.9.SPINE1235
http://dspace.inha.ac.kr/handle/10505/31562
ISSN
1547-5654
Appears in Collections:
Medical School/College of Medicine (의학전문대학원/의과대학) > Medical Science (의학) > Journal Papers, Reports(의학 논문, 보고서)

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