Construction of the first compendium of chemical-genetic profiles in the fission yeast Schizosaccharomyces pombe and comparative compendium approach.

Title
Construction of the first compendium of chemical-genetic profiles in the fission yeast Schizosaccharomyces pombe and comparative compendium approach.
Authors
황성욱
Keywords
Chemical-genetic profile Budding yeast Fission yeast Drug-induced haploinsufficiency
Issue Date
2013
Publisher
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Series/Report no.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ; Vol.436 no.4 Startpage 613 Endpage 618
Abstract
Genome-wide chemical genetic profiles in Saccharomyces cerevisiae since the budding yeast deletion library construction have been successfully used to reveal unknown mode-of-actions of drugs. Here, we introduce comparative approach to infer drug target proteins more accurately using two compendiums of chemical-genetic profiles from the budding yeast S. cerevisiae and the fission yeast Schizosaccharomyces pombe. For the first time, we established DNA-chip based growth defect measurement of genome-wide deletion strains of S. pombe, and then applied 47 drugs to the pooled heterozygous deletion strains to generate chemical-genetic profiles in S. pombe. In our approach, putative drug targets were inferred from strains hypersensitive to given drugs by analyzing S. pombe and S. cerevisiae compendiums. Notably, many evidences in the literature revealed that the inferred target genes of fungicide and bactericide identified by such comparative approach are in fact the direct targets. Furthermore, by filtering out the genes with no essentiality, the multi-drug sensitivity genes, and the genes with less eukaryotic conservation, we created a set of drug target gene candidates that are expected to be directly affected by a given drug in human cells. Our study demonstrated that it is highly beneficial to construct the multiple compendiums of chemical genetic profiles using many different species. The fission yeast chemicalgenetic compendium is available at http://pombe.kaist.ac.kr/compendium.
URI
http://dx.doi.org/10.1016/j.bbrc.2013.05.138
http://dspace.inha.ac.kr/handle/10505/31550
ISSN
0006-291X
Appears in Collections:
Medical School/College of Medicine (의학전문대학원/의과대학) > Medical Science (의학) > Journal Papers, Reports(의학 논문, 보고서)
Files in This Item:
33168.pdfDownload

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse