SPT4 increases UV-induced mutagenesis in yeast through impaired nucleotide excision repair

Title
SPT4 increases UV-induced mutagenesis in yeast through impaired nucleotide excision repair
Authors
이성근
Keywords
SPT4, RAD2, Transcription elongation, Mutagenesis, DNA repair
Issue Date
2013
Publisher
MOLECULAR & CELLULAR TOXICOLOGY
Series/Report no.
MOLECULAR & CELLULAR TOXICOLOGY ; Vol.9 no.1 Startpage 37 Endpage 43
Abstract
DNA repair is a pivotal mechanism in maintaining genetic integrity and cell fate determination. As unrepaired DNA lesions inhibit transcription, UV-induced damage to transcribed DNA is repaired preferentially versus non-transcribed DNA through transcription-coupled nucleotide excision repair (TCR). Previously, we reported that TCR-related genes serve as transcription elongation factors, and defects of the genes drastically increase mutagenesis. Extensive studies on DNA damage repair have provided key information about the pathways controlling replication across DNA lesions. However, knowledge of the mechanisms dealing with stalled DNA transcription is insufficient. In this study, we demonstrated the requirement for SPT4 in cell growth along with its role in mutagenesis in both the presence and absence of DNA damage. SPT4 appeared to promote transcription elongation across DNA lesions, thereby increasing the cell survival rate in exchange for increased mutagenesis. Further, our results explain the decrease in mutant Huntingtin protein in neuronal cells upon inhibition of Supt4, the mammalian ortholog of yeast Spt4p.
URI
http://dspace.inha.ac.kr/handle/10505/31306
ISSN
1738-642X
Appears in Collections:
Medical School/College of Medicine (의학전문대학원/의과대학) > Medical Science (의학) > Journal Papers, Reports(의학 논문, 보고서)
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