Effect of Two Lipid Emulsions on Reversing High-Dose Levobupivacaine-Induced Reduced Vasoconstriction in the Rat Aortas

Title
Effect of Two Lipid Emulsions on Reversing High-Dose Levobupivacaine-Induced Reduced Vasoconstriction in the Rat Aortas
Authors
박창신
Keywords
Lipid emulsion Levobupivacaine Nitric oxide Vasoconstriction Aorta
Issue Date
2013
Publisher
CARDIOVASCULAR TOXICOLOGY
Series/Report no.
CARDIOVASCULAR TOXICOLOGY ; Vol.13 no.4 Startpage 370 Endpage 380
Abstract
The goals of this study were to determine which lipid emulsion (Intralipid and Lipofundin MCT/ LCT) is more effective in reversing high-dose levobupivacaine-induced reduced vasoconstriction in isolated rat aortas and to examine the associated cellular mechanisms with a particular focus on the endothelium. Two lipid emulsion concentration?response curves were generated using high-dose levobupivacaine-induced reduced vasoconstriction and vasodilation of isolated aortas pretreated with or without 60 mM KCl. Endothelial nitric oxide synthase (eNOS) and caveolin-1 phosphorylation were measured in rat aortic tissue treated with levobupivacaine in the presence or absence of lipid emulsion. Dichlorofluorescein oxidation, a measure of reactive oxygen species production, was measured in lipid emulsion-treated human umbilical vein endothelial cells. In levobupivacaine (0.3 mM)-induced reduced vasoconstriction of isolated aorta, the magnitude of the Intralipid- and Lipofundin MCT/LCT-mediated reversal was not significantly different. Lipid emulsion reversal of levobupivacaine-induced reduced vasoconstriction was greater in endothelium-intact aortas than in endothelium-denuded aortas. The two lipid emulsions similarly inhibited levobupivacaine-induced eNOS phosphorylation in aortic tissue. Pretreatment with both lipid emulsions increased dichlorofluorescein oxidation. Both Intralipid and Lipofundin MCT/LCT are equally effective for vascular tone recovery from high-dose levobupivacaine-induced reduced vasoconstriction. This reversal is mediated partially by decreasing nitric oxide bioavailability.
URI
http://dspace.inha.ac.kr/handle/10505/31300
ISSN
1530-7905
Appears in Collections:
Medical School/College of Medicine (의학전문대학원/의과대학) > Medical Science (의학) > Journal Papers, Reports(의학 논문, 보고서)
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