A guanidinylated bioreducible polymer as a novel gene carrier to the corpus cavernosum of mice with high-cholesterol diet-induced erectile dysfunction.

Title
A guanidinylated bioreducible polymer as a novel gene carrier to the corpus cavernosum of mice with high-cholesterol diet-induced erectile dysfunction.
Authors
서준규
Keywords
bioreducible polymer, erectile dysfunction, gene therapy, guanidinylation, non-viral vector
Issue Date
2013
Publisher
ANDROLOGY
Series/Report no.
ANDROLOGY ; Vol.1 no.2 Startpage 216 Endpage 222
Abstract
A prerequisite for the successful clinical application of gene therapy in erectile dysfunction (ED) is the availability of safe and effieient gene delivery systems. The aim of this study was to examine the effeetiveness of guanidinylated hioredueihle polymer (GBP) polyplexes for gene delivery systems, whieh take advantage of the biodegradahility of redueihle disulfide honds and the eell-penetrating ability of guanidine groups. Eor in vitro transfection experiments, we used mouse cavernous endothelial cells and A7r5 rat vascular smooth muscle cells. Eor in vivo experiments, we used a mouse model of hypercholesterolaemie ED in which 2-month-old male C57BL/6 mice were fed a diet containing 4% cholesterol and 1% cholic acid for 3 months. Animals or cells were treated with pCMVLue, poly(ethyleneimine) (PEI)25k/pCMV-Luc polyplex (weight ratio; 1) and GBP/pCMV-Luc polyplexes (weight ratio; 20, 40, 60 and 80). Gene expression was evaluated by luciferase assay, and the gene expression area was
URI
http://dspace.inha.ac.kr/handle/10505/31285
ISSN
2047-2919
Appears in Collections:
Medical School/College of Medicine (의학전문대학원/의과대학) > Medical Science (의학) > Journal Papers, Reports(의학 논문, 보고서)

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