Calcium phosphate cements loaded with basic fibroblast growth factor: Delivery and in vitro cell response

Title
Calcium phosphate cements loaded with basic fibroblast growth factor: Delivery and in vitro cell response
Authors
장준혁
Keywords
calcium phosphate cements, powder size, growthfactor delivery, cell proliferation, bone regeneration
Issue Date
2013
Publisher
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Series/Report no.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A ; Vol.101A no.4 Startpage 923 Endpage 931
Abstract
Combining calcium phosphate cements (CPCs) withbioactive molecules improves their bone regeneration poten-tial. Although CPCs are highly osteoconductive, sometimesthey have limited biological responses, especially in terms ofcell proliferation. Here, we used basic fibroblast growth factor(bFGF) in an a-tricalcium phosphate cement with different ini-tial powder sizes (coarse vs. fine; designated as CPC-C andCPC-F, respectively) and investigated the behavior of bFGFloading and release, as well as the effects on osteoblastresponses. bFGF was loaded at 10 lg/ml or 25 lg/ml onto theset form of two types of CPCs, aiming to allow penetrationinto the pore structure and adsorption onto the cement crystal-lites. The CPC formulated with fine powder (CPC-F) had higherspecific surface area and smaller-sized pores and retainedslightly higher amounts of bFGF within the structure. ThebFGF release study performed for 3 weeks showed a sus-tained-release profile; after an initial rapid release over approx-imately 3 days, further release pattern was almost linear.Compared to CPC-F, CPC-C showed a much faster release pat-tern. The effects of the bFGF incorporation within CPCs on cel-lular responses were assessed in terms of cell proliferationusing MC3T3-E1 pre-osteoblastic cells. Compared with bFGF-free CPCs (both CPC-C and CPC-F), those containing bFGFstimulated cell proliferation for up to 7 days. An inhibitionstudy of bFGF receptor demonstrated that the improvement ofcell proliferation resulted from the role of bFGF released fromthe CPCs. This study provides beneficial information onimproving the biological properties of CPCs by combiningthem with specific therapeutic molecules, and particularly withbFGF, showing that the cell proliferative ability was signifi-cantly stimulated, which may have potential applications forfurther use in stem cell-based bone tissue engineering.
URI
http://dspace.inha.ac.kr/handle/10505/31196
ISSN
1549-3296
Appears in Collections:
Medical School/College of Medicine (의학전문대학원/의과대학) > Medical Science (의학) > Journal Papers, Reports(의학 논문, 보고서)

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