Tethering bi-functional protein onto mineralized polymer scaffolds to regulate mesenchymal stem cell behaviors for bone regeneration

Title
Tethering bi-functional protein onto mineralized polymer scaffolds to regulate mesenchymal stem cell behaviors for bone regeneration
Authors
장준혁
Issue Date
2013
Publisher
JOURNAL OF MATERIALS CHEMISTRY B
Series/Report no.
JOURNAL OF MATERIALS CHEMISTRY B ; Vol.1 no.21 Startpage 2731 Endpage 2741
Abstract
Modifying three-dimensional scaffolds with bioactive extracellular matrix (ECM) molecules enhances their potential use in tissue engineering, by providing natural biochemical/physical cues for cell recognition. Here, we engineered the surface of poly(caprolactone) (PCL) scaffolds, first with bone mineral hydroxyapatite (HA), and then with fibronectin?osteocalcin (FN?OCN) bi-functional protein by means of affinity binding between OCN and HA. While FN is expected to enhance initial adhesion of immature precursor cells, OCN is considered to regulate osteogenic differentiation. Quartz crystal microbalance dissipation analysis revealed FN?OCN protein had a more stable and stronger adherence to the HA-mineralized surface than to the native PCL-surface. Initial adhesion and the spreading of rat mesenchymal stem cells were significantly enhanced on the FN?OCN tethered scaffold. Expression of bone-associated genes (osteopontin, bone sialoprotein II and OCN) was significantly higher on the FN?OCN tethered scaffold. Moreover, those proteins were more abundantly found when cultured on the scaffolds with FN?OCN than those without, as confirmed by immunofluorescence cell labeling and fluorescence activated cell sorting analysis. All taken, the tethering of FN?OCN to a HA-mineralized surface is an effective strategy to provide biopolymer scaffolds improved bi-functional capacity for bone tissue engineering, in terms of initial cell adhesion and osteogenic differentiation.
URI
http://dspace.inha.ac.kr/handle/10505/31195
ISSN
0959-9428
Appears in Collections:
Medical School/College of Medicine (의학전문대학원/의과대학) > Medical Science (의학) > Journal Papers, Reports(의학 논문, 보고서)
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