Mineralized poly(lactic acid) scaffolds loading vascular endothelial growth factor and the in vivo performance in rat subcutaneous model

Title
Mineralized poly(lactic acid) scaffolds loading vascular endothelial growth factor and the in vivo performance in rat subcutaneous model
Authors
장준혁
Keywords
PLA scaffold, VEGF, CaP mineralization, surfacemodification, rat subcutaneous model
Issue Date
2013
Publisher
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Series/Report no.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A ; Vol.101A no.5 Startpage 1447 Endpage 1455
Abstract
The functionalization of degradable polymeric scaf-folds with therapeutic molecules such as vascular endothelialgrowth factor (VEGF) is a key strategy to gain better regener-ative ability of damaged bone tissue by stimulating vasculari-zation and tissue perfusion. Here, we combined VEGF withpoly(lactic acid) (PLA) porous scaffold, after modifying thePLA surface with calcium phosphate (CaP) mineral. The min-eralized PLA scaffold (mPLA) showed more effective loadingcapacity of VEGF than the PLA without mineralization as wellas profiled sustainable release of VEGF for up to a couple ofweeks. The VEGF-loaded mPLA scaffold presented signifi-cantly improved proliferation of primary endothelial cells forup to 7 days, with respect to the scaffold without the VEGFloading. The performance of the engineered scaffold wasassessed after subcutaneous implantation in rats for 4 weeks.Histological results showed favorable tissue compatibility ofboth the mPLA scaffolds (with and without VEGF loading), ascharacterized by infiltration of inflammatory cells, formationof fibrous capsule, and ingrowth of fibroblasts into the matri-ces. Immunohistochemical staining of the von Willebrand Fac-tor revealed significantly improved formation of neo-capillaries in the VEGF-loaded mPLA. Based on this study, thestrategy of VEGF loading onto mineralized PLA scaffold is con-sidered beneficial for gaining improved vascularization of thepolymeric scaffolds, suggesting potential applications forbone tissue engineering.
URI
http://dspace.inha.ac.kr/handle/10505/31190
ISSN
1549-3296
Appears in Collections:
Medical School/College of Medicine (의학전문대학원/의과대학) > Medical Science (의학) > Journal Papers, Reports(의학 논문, 보고서)

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