Glucosamine inhibits lipopolysaccharide-stimulated inducible nitric oxide synthase induction by inhibiting expression of NF-kappaB/Rel proteins at the mRNA and protein levels.

Title
Glucosamine inhibits lipopolysaccharide-stimulated inducible nitric oxide synthase induction by inhibiting expression of NF-kappaB/Rel proteins at the mRNA and protein levels.
Authors
한인옥
Keywords
Glucosamine Inflammation LPS iNOS NF-jB
Issue Date
2013
Publisher
NITRIC OXIDE
Series/Report no.
NITRIC OXIDE ; Vol.31 Startpage 1 Endpage 8
Abstract
Expression of inducible nitric oxide synthase (iNOS) protein by lipopo lysaccharide (LPS) in BV2 mic roglia cells increased in a biphasic manner. Glucosamine (GlcN) selectively suppressed the late- but not earlystage iNOS response to LPS. Prolonged induction of iNOS expression by LPS was inhibite d by cycloheximide, suggesting that de novo protein synthesis was required. Late-phase activation of nuclear factorkappaB (NF-jB) activity required for sustained iNOS induction. Nuclear translocation and DNA binding of NF- jB, and Rel proteins expressions were inhibited by GlcN at later time points but not upon immediate early-stage activa tion by LPS. We show that GlcN selectively inhibits sustained iNOS induction by inhibiting Rel protein expression at both the mRNA and protein levels; such expression is required for prolonged iNOS induction by LPS. Our results provide mechanistic evidence that GlcN regulates inflammation, represented by iNOS. The implication of these results is that GlcN may be a potent transcriptional regulator of iNOS and other genes involved in the general inflammation process.
URI
http://dx.doi.org/10.1016/j.niox.2013.02.082
http://dspace.inha.ac.kr/handle/10505/31183
ISSN
1089-8603
Appears in Collections:
Medical School/College of Medicine (의학전문대학원/의과대학) > Medical Science (의학) > Journal Papers, Reports(의학 논문, 보고서)
Files in This Item:
32802.pdfDownload

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse