Characterization and optimization of vascular endothelial growth factor(165) (rhVEGF(165)) expression in Escherichia coli.

Title
Characterization and optimization of vascular endothelial growth factor(165) (rhVEGF(165)) expression in Escherichia coli.
Authors
서창국
Keywords
Vascular endothelial growth factor Protein expression Calf pulmonary artery endothelial cell Cell proliferative activity Gene expression
Issue Date
2013
Publisher
PROTEIN EXPRESSION AND PURIFICATION
Series/Report no.
PROTEIN EXPRESSION AND PURIFICATION ; Vol.87 no.2 Startpage 55 Endpage 60
Abstract
Vascular endothelial growth factors165 (VEGF165) is the most potent and widely used pro-angiogenic factor. Here we determined optimal culture condition of recombinant human VEGF165 (rhVEGF165) in Escherichia coli(E. coli). rhVEGF165 expression was the highest in 0.25% of L-arabinose induction concentration, at 20 C induction temperature, and for 5 h induction time under the control of araBAD promoter using pBADHisA vector. In biological activity test, rhVEGF165 significantly increased the proliferative activity of CPAE cells (p < 0.001) and upregulated the expressions of endothelial cell growth-related genes, such as platelet endothelial cell adhesion molecule (PECAM-1), endothelial-specific receptor tyrosine kinase (TEK), kinase insert domain protein receptor (KDR), and tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1) in calf pulmonary artery endothelial (CPAE) cells.
URI
http://dx.doi.org/10.1016/j.pep.2012.10.004
http://dspace.inha.ac.kr/handle/10505/31177
ISSN
1046-5928
Appears in Collections:
Medical School/College of Medicine (의학전문대학원/의과대학) > Medical Science (의학) > Journal Papers, Reports(의학 논문, 보고서)
Files in This Item:
32796.pdfDownload

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse