효소 모방 자성나노촉매를 이용한 p-Nitrophenyl alkyl esters 고효율 분해

Title
효소 모방 자성나노촉매를 이용한 p-Nitrophenyl alkyl esters 고효율 분해
Authors
이선영
Keywords
효소모방자성나노촉매를이용한pnitrophenylalkylesters고효율분해
Issue Date
2012
Publisher
인하대학교
Abstract
This work reports the development of the high throughput hydrolysis of p-nitrophenyl alkyl esters(PNPE) with enzyme-mimic catalysts which are originated from silica-coated magnetic nanoparticles(Si-MNPs). Si-MNPs were prepared by coprecipitation of Fe2+ and Fe3+, and then tetraethyl orthosilicate(TEOS) was directly coated by sol-gel process. Functional groups such as amine, carboxyl, imidazole groups were grafted on the Si-MNPs surface mimicking a triads of Ser 195, His 57, Asp 102 of chymotrypsin. These groups were required for enhanced catalytic activity toward the hydrolysis of ester bond of substrates. To compare catalytic activity of enzyme-mimic Si-MNPs with chymotrypsin, hydrolysis of PNPE were observed by UV-visible spectrophotometer. Catalytic activities were obtained using Michaelis-Menten kinetics. The result showed that the hydrolysing property of enzyme-mimic Si-MNPs were catalytic efficiency such as chymotrypsin. It suggests that the enzyme-mimic Si-MNPs might be used for biosensors, biocatalysts or proteome research.
Description
목 차 Abstract Korean of Abstract List of Figures List of Table Nomenclaturese 1. 서 론 2. 이 론 2.1. 자성나노입자(Magnetic nanoparticles, MNPs) 2.2. 카이모트립신(Chymotrypsin) 2.3. 미카엘리스-멘텐 방정식(Michaelis-Menten equation) 3. 실험 방법 3.1. 시약과 분석기기 3.1.1. 시약 3.1.2. 분석기기 3.2. 자성나노입자의 합성 3.3. 실리카 코팅된 자성나노입자의 합성 3.4. 효소 모방 자성나노촉매의 합성 3.5. p-Nitrophenyl alkyl esters(PNPE)의 가수분해 3.5.1. 0.1M Tris-buffer의 제조 3.5.2. PNPE solution의 제조 3.5.3. 카이모트립신의 PNPE 가수분해 3.5.4. 효소 모방 자성나노촉매의 PNPE 가수분해 4. 결과 및 토의 4.1. 자성나노입자와 실리카 코팅된 자성나노입자의 특성 4.2. 효소 모방 자성나노촉매의 특성 4.3. 효소 모방 자성나노촉매의 효율 5. 결 론 참고 문헌 List of Figures Figure 1. Scheme of active site of chymotrypsin. Figure 2. Steps in the hydrolysis of p-Nitrophenyl esters. Figure 3. Plot of initial velocity as a function of substrate concentration with significance of the kinetic parameters Vmax and Km graphically depicted. Figure 4. Synthesis process of magnetic nanop
URI
http://dspace.inha.ac.kr/handle/10505/23678
Appears in Collections:
College of Natural Science(자연과학대학) > Chemistry (화학) > Theses(화학 석박사 학위논문)
Files in This Item:
24789.pdfDownload

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse