Astrocyte evaluation on the electrospun PCL nanofiber mat containing the brown seaweed EPS

Title
Astrocyte evaluation on the electrospun PCL nanofiber mat containing the brown seaweed EPS
Authors
김성훈
Keywords
astrocyteevaluationontheelectrospunpclnanofibermatcontainingthebrownseaweedeps
Issue Date
2012
Publisher
인하대학교
Abstract
Central nervous system (CNS), once injured, hardly recover original functions mainly due to its limited regeneration ability. Several biomaterials have been tried to replace and regenerate lost tissues of injured CNS. Seaweeds have extracellular polymeric substances (EPS) to have bioactive properties including antiviral and antioxidant activities. In this research, astrocyte activity was assessed once cultured on an electrospun polycaprolacton (PCL) nanofibrous mat containing brown seaweed EPS, which has been known to be enhanced as CNS is injured. EPS extracts was observed to be released from PCL-seaweed EPS nanofiber during cultivation of astrocytes and were proven to rarely influence growth and metabolic activity of rat primary astrocytes without cytotoxic effects. This implies that seaweed EPS-PCL nanofiber mat does not enhance astrogliosis which is one of main reasons of glia scar formation in the injured site of CNS, where supplying bioactive EPS extracts to. Through this research, we first propose that an electrospun seaweed EPS-PCL nanofiber could be used to treat CNS injury.
Description
1. Introduction 1 1.1 Central nervous system (CNS) 1 1.1.1 Central nervous system (CNS) 1 1.1.2 Spinal cord injury (SCI) 1 1.1.3 Astrocyte 2 1.2 Electrospun nanofiber 3 1.3 Extracellular polymeric substance (EPS) 3 2. Research purpose 4 3. Materials and methods 4 3.1 Isolation of rat primary astrocyte 5 3.2 EPS extraction 6 3.3 Fabrication of electrospun nanofiber 7 3.4 Characterization 8 3.4.1 Morphology of electrospun nanofiber 8 3.4.2 Contact angle analysis 8 3.4.3 Mechanical strength analysis 9 3.4.4 Thermogravimetric analysis 9 3.5 Cell culture test 10 3.5.1 MTT assay 10 3.5.2 Biochemical effect of EPS on astrocyte 10 3.5.3 Cytotoxicity evaluation of PCL-EPS nanofiber mat 11 3.5.4 Cell adhesion assay 11 3.5.5 Viability assay 12 3.5.6 GFAP expression analysis 12 3.6 Statistical analysis 13 4. Results and Discussions 14 4.1 Mechanical properties of the electrospun nanofiber containing EPS 14 4.2 Biochemical effect of EPS on astrocytes 15 4.3 Release of EPS from the electrospun nanofiber containing EPS 17 4.4 Cytotoxicity of PCL-EPS nanofiber 18 4.5 Physicobiochemical effect of PCL-EPS nanofiber on astrocyte adhesion 19 4.6 Physicobiochemical effect of PCL-EPS nanofiber on astrocyte viability 20 4.7 GFAP analysis 20 5. Conclusion 22 6. References 24
URI
http://dspace.inha.ac.kr/handle/10505/23647
Appears in Collections:
College of Natural Science(자연과학대학) > Ocean Sciences (해양과학) > Theses(해양과학 석박사 학위논문)
Files in This Item:
24728.pdfDownload

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse