항염증 및 항산화 직용에 의한 에틸파이루베이트의 신경세포 보호효과

Title
항염증 및 항산화 직용에 의한 에틸파이루베이트의 신경세포 보호효과
Authors
신주현
Keywords
항염증및항산화직용에의한에틸파이루베이트의신경세포보호효과
Issue Date
2012
Publisher
인하대학교
Abstract
Ethyl pyruvate (EP), a simple ester of pyruvic acid, has been shown to have protective effect under various pathological conditions, such as sepsis, endotoxemia, acute pancreatitis, and cerebral ischemia. During cerebral ischemia, oxidative stress and inflammation play critical roles in aggravating the delayed damaging process as well as the excitotoxicity-induced acute damaging process. Astrocytes are known to support the protection and survival of neurons against inflammation and oxidative damage. In order to verify the molecular mechanism underlying the anti-oxidative effect of EP in astrocyte, the activation of nuclear component E2-related factor 2 (Nrf2), a master transcriptional regulator of phase II antioxidative genes, was examined. EP was found to induce the Nrf2 translocation and upregulations of various genes downstream of Nrf2, such as hemeoxygenase 1 (HO-1), and these Nrf2 activation and HO-1 induction resulted in the amelioration of H2O2-induced oxidative damage in prima
Description
List of Contents Abstract i Abstract in Korean iv List of Contentsvi List of Figures ix I. Introduction 1 II. Materials and Methods 8 1. Primary astrocyte culture and H2O2 treatment 2. Cortical neuron-enriched cultures and treatment with H2O2, Zn2+, and Fe2+ 3. Primary microglia culture and cell culture 4. Ethyl pyruvate and sodium pyruvate treatment 5. Immunoblotting and co-immunoprecipitation analysis 6. Measurement of cell viability and cytotoxicity assay 7. siRNA transfection 8. Treatments of ZnPP, PD98059, and wortmannin 9. Plasmid transfection and luciferase assays 10. Electrophoretic mobility shift assays 11. Immunocytochemistry 12. Ethyl pyruvate conditioned medium and GDNF production assay 13. Treatment of recombinant GDNF 14. Surgical procedures for MCA occlusion and CSF sampling 15. Infarct volume assessment 16. Ethyl pyruvate and A23187 treatment 17. Preparation of cell extracts and protein contents in media 18. Calcium assay and staining 19. Statistical Analysis III. Re
URI
http://dspace.inha.ac.kr/handle/10505/23474
Appears in Collections:
Medical School/College of Medicine (의학전문대학원/의과대학) > Medical Science (의학) > Theses(의학 석박사 학위논문)
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