생체환경 모사 및 세포 반응 분석을 위한 미세시스템 개발

Title
생체환경 모사 및 세포 반응 분석을 위한 미세시스템 개발
Authors
유사무엘
Keywords
생체환경모사및세포반응분석을위한미세시스템개발
Issue Date
2012
Publisher
인하대학교
Abstract
In this study, we developed a microsystem for mimicking in vivo environments and analyzing cell responses. First, patterns with micro/nano-topographical structures were studied to mimic in vivo environments. Different types of micro/nanostructures were used as a cell culture substrate to maintain innate cell functions. Micro/nano-patterns were simply created on Polydimethylsiloxane (PDMS) by stretching a PDMS mold exposed to plasma. Three different types of cells were cultured for 1 day on the patterns. After seeded, cells were observed by phase contrast microscopy to analyze cell morphology, adhesion, and alignment. Cells on the deeper pattern were more fibrous and aligned along the pattern. Second, a micromixer for the analysis of cell responses to various biochemical agents was studied. Efficient mixing is needed for the rapid and accurate analysis of responses. But fluids in microscale are restricted to laminar flow, resulting in poor mixing. The micromixer developed in this work is based on the non-equilibrium electrokinetics at the junction of a micro/nano-channel. Two fluids were mixed by an electroosmotic flow and a vortex flow created by non-equilibrium electrokinetics at the junction of the micro/nano-channel. The microchannel was fabricated with PDMS by a general soft lithography process and the nanochannel was created at the specific position on the microchannel by using the electrical breakdown of PDMS with high electrical voltage. To evaluate the mixing performance of the micromixer, the distribution of fluorescent intensity was analyzed by using fluorescent dye, Rhodamine B. Approximately 90% mixing of two fluids was achieved with our novel micromixer. This study can be helpful for the development of a biomimetic microsystem for analyzing cell responses.
Description
제 1 장 서 론 1 제 2 장 실험 방법 6 2.1 단순공정을 통한 생체모사 미세패턴 제작 6 2.1.1 PDMS 표면 개질 및 미세패턴 제작 6 2.1.2 PDMS 표면에 형성된 미세패턴의 크기 분석 7 2.2 생체모사 미세패턴을 이용한 세포 접촉 유도 8 2.2.1 재료 및 시약 8 2.2.2 세포 배양 방법 10 2.2.3 미세패턴의 세포 접촉 유도영향 분석 11 2.3 유해물질 검측을 위한 미세혼합기 제작 12 2.3.1 소프트리소그래피 공정을 이용한 미세혼합기 제작 12 2.3.2 전기적 파괴를 이용한 나노채널 제작 14 2.3.3 미세채널 내 저항 측정을 통한 나노채널 형성 검증 14 2.3.4 미세/나노채널 부의 불균형 동전기성을 이용한 혼합실험 16 제 3 장 결과 및 논의 17 3.1 단순공정으로 제작된 생체모사 미세패턴 형성 17 3.1.1 단순공정으로 제작된 미세패턴 분석 17 3.2 생체모사 미세패턴을 이용한 세포 접촉 유도 19 3.2.1 미세패턴 크기에 따른 세포 성장형태 및 유도체의 영향 19 3.2.2 세포 접촉력 및 유도 각도 분석 20 3.3 유해물질 검측을 위한 미세혼합기 21 3.3.1 PDMS의 전기적 파괴에 의한 나노채널 형성 21 3.3.2 미세혼합기의 혼합 성능 22 제 4 장 결 론 26 참고문헌 48
URI
http://dspace.inha.ac.kr/handle/10505/23327
Appears in Collections:
College of Engineering(공과대학) > Mechanical Engineering(기계공학) > Theses(기계공학 석박사 학위논문)
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