Purification of Lactic Acid and Astaxanthin by Specific Stationary Phases

Title
Purification of Lactic Acid and Astaxanthin by Specific Stationary Phases
Authors
주군
Keywords
purificationoflacticacidandastaxanthinbyspecificstationaryphases
Issue Date
2011
Publisher
인하대학교
Abstract
Lactic acid, as a versatile chemical, was purified from fermentation broth by solid phase extraction with a spherical porous poly(4-vinylpyridine). Adsorption isotherm of lactic acid on poly(4-vinylpyridine) was firstly investigated with model solutions. The factors which affect the performance of separation and purification were then investigated. The obtained results indicate that the sorbent had a high adsorption capability of lactic acid, of which the maximum adsorbed lactic acid was 180.0 mg/g. Under the optimal condition the lactic acid was purified from fermentation broth by solid phase extraction with the purity of 88% and recovery yield of 95%. Also, another specific kind of stationary phases (ionic liquid-based silicas) were prepared to separate and determine astaxanthin content in Saccharina japonica. The optimized process included extraction, saponification and purification. The optimal extraction conditions with different solvents were investigated. 29.30 ?g/g of astaxanthin was extracted from dry Saccharina japonica powder by solvent. After subsequent saponification, the extracted amount of astaxanthin was increased to 37.26 ?g/g. Furthermore, three different ionic liquid-based silicas were prepared as sorbents for the solid phase extraction of astaxanthin from the extract. By comparing the adsorption isotherms of astaxanthin on different ionic liquid-based silicas, suitable sorbent was successfully selected and applied for separation of astaxanthin from extract solution.
Description
1. Introduction 1 1.1 Lactic acid 1 1.2 Astaxanthin 2 2. Theoretical background 5 2.1 Retention mechanisms 5 2.1.1 Exothermodynamic relationships 5 2.1.2 Analysis of selectivity 6 2.2 Solid-phase extraction (SPE) 8 3. Experimental 9 3.1 Chemicals 9 3.1.1 Lactic acid 9 3.1.2 Astaxanthin 10 3.2 HPLC analysis 10 3.2.1 Lactic acid 10 3.2.2 Astaxanthin 11 3.3 Methods 11 3.3.1 Purification of lactic acid by spherical anion exchange polymer 12 3.3.2 Purification of astaxanthin by ionic liquid-modified silicas 14 4. Results and discussion 18 4.1 Purification of lactic acid by spherical anion exchange polymer 18 4.1.1 Performance evaluation 18 4.1.2 Adsorption isotherm 19 4.1.3 Optimization of SPE conditions 21 4.1.4. Validation of proposed analytical method 25 4.2 Purification of astaxanthin by ionic liquid-modified silicas 25 4.2.1 Optimization of extraction and saponification conditions 26 4.2.2 Optimization of SPE conditions 29 4.2.3 Validation of proposed analytical method 33 5. Conclusions 34 Summary in Korean 36 Summary in Chinese 37 References 38 Appendix 1. Extraction and separation of (D, L)-lactic acid in simulated fermentation broth 42 Appendix 2. Separation of xylose and glucose on different silica-confined ionic liquid stationary phases 43 Appendix 3. Solid phase extraction of lactic acid from fermentation broth by anion exchangeable silica confined ionic liquids 44 Appendix 4. Task-specific ionic liquid-assisted extraction and separation of astaxanthin from shrimp waste 45 Appendix 5. Decaffeination of coffee bean waste by solid-liquid extraction 46 List of Publications 47 Table 1. Contents of total astaxanthin in various sources. 3 Table 2. Parameters in adsorption isotherm equations of lactic acid on the P4VP. 21 Table 3. RSDs and LOD of lactic acid. 25 Table 4.Parameters in adsorption isotherms of AX on different Si-ILs. 32 Figure 1.Structures of astaxanthin. . 2 Figure
URI
http://dspace.inha.ac.kr/handle/10505/22889
Appears in Collections:
College of Engineering(공과대학) > Chemical Engineering (화학공학) > Theses(화학공학 석박사 학위논문)
Files in This Item:
23946.pdfDownload

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse