해양 천연물에서 다당류의 추출 최적화

Title
해양 천연물에서 다당류의 추출 최적화
Authors
허효정
Keywords
해양천연물에서다당류의추출최적화
Issue Date
2011
Publisher
인하대학교
Abstract
Response surface methodology (RSM) was used to optimize extraction conditions for polysaccharides from Hizikia fusiforme (H. fusiforme) and Undaria pinnatifida (U. pinnatifida). Three independent variables, extraction time, temperature and ratio of water to raw material were investigated for optimization of extraction conditions by a Box-Behnken design (BBD) and central composite design (CCD). The experimental data were fitted to a quadratic polynomial model using multiple regression analysis and were examined using suitable statistical methods. In the work of extraction of H. fusiforme, the adjusted coefficient of determination (r2Adj) for the model was 0.93, and the probability value (p = 0.0002) demonstrated a high significance for the regression model. The optimum extraction conditions were found to be: optimized extraction time 2.05 h, extraction temperature 75.45 ℃, and ratio of water to raw material 29.89. Under these conditions, the mean extraction yield of polysaccharides was 21.83 %, which was in good conformance with the predicted model value. In the experiment of extraction of U. pinnatifida, the value of r2Adj for the model was 0.92. The optimum extraction yield (30.78 %) was obtained, when the extraction temperature was 95 ℃, extraction time was 4.5 h, ratio of water to raw material was 35. Under these conditions, validation experiments were done and the mean extraction yield of polysaccharides was 30.79 %, which was in good agreement with the predicted model value. Ionic liquids can be used as mobile phase additives in reversed-phase chromatography when mixed with other solvents such as methanol and acetonitrile. The addition of ionic liquids could change the retention factors of the analytes, decreases the band tailing, reduces the band broadening, and improves the resolution partly because of the competition between the imidazolium cations and the polar groups of the analyte for the silanol group on the alkyl silica surface along with the formation
Description
Table of Contents ABSTRACT Table of Contents List of Tables List of Figures 1. Introduction 2. Theoretical background 2.1. Experimental design 2.2. Correlations for retention factor 3. Experimental 3.1. Chemicals 3.2. Apparatus 3.3. Extraction of polysaccharides 3.4. Analytical condition of UV spectroscopy 3.5. Analytical condition of HPLC 4. Results and discussions 4.1. Model fitting 4.2. Statistical analysis in modeling 4.2.1. H. fusiformis 4.2.2. U. pinnatifida 4.4. Optimization of the procedure 4.4.1. BBD model of H. fusiformis 4.4.2. CCD model of U. pinnatifida 4.5. Validation of the model 4.6. Effect of Ionic liquids on retention factor 4.6.1. Correlation equation 4.6.2. Effect of ionic liquids on the basic compounds 5. Conclusion 한글요약 References Appendix 1. Cleanliness Test by Spray-Type Cleaning Agent for Electronic and Semiconductor Equipment Appendix 2. Evaluation of Cleanliness and Jet Forces by Spray-Type Cleaning Agent for Electronic and miconductor Equipment List of Publications 감사의 글
URI
http://dspace.inha.ac.kr/handle/10505/22884
Appears in Collections:
College of Engineering(공과대학) > Chemical Engineering (화학공학) > Theses(화학공학 석박사 학위논문)
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