Characterization of Flavonol Glycosides Synthesized by Enzymatic Hydrolysis of Tea Seed Extract

Characterization of Flavonol Glycosides Synthesized by Enzymatic Hydrolysis of Tea Seed Extract
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Flavonoid containing plant extracts have been widely used as topical applications for wound-healing, anti-aging, and disease treatments. Since the cancer-protective effect of drinking green tea was reported, much research has been focused on the isolation and biological effect of each component contained in tea, especially polyphenols. The major poly-phenols present in tea are flavan-3-ols and flavonols. Flavan-3-ols account for about 77% of phenolic compounds in tea and flavonols comprise about 13%. However, flavanols are structurally less stable than flavonols. The major flvonols in tea are glycosides of quercetin or kaempferol. As the popularity of tea increases, production of tea seed has also increased. Tea seed also contains many biologically active compounds such as saponin, flavonoid, vitamins, and tannins. Concerning the commercial use of flavonols for other industrial purposes, the production of pure separated flavonols from plant sources such as tea is not easy. In this study, to isolation and characterization of flavonol glycosides synthesized by enzymatic hydrolysis of tea seed extract, two flavonol triglycosides, camelliaside A (CamA) and camelliaside B (CamB) of tea seed extract were subjected to enzymatic hydrolysis. In order to produce of flavonol diglycoside, the combination of the use of Gal and Pec in the enzymatic hydrolysis of TSE afforded NF with high specificity. β-galactosidase (Gal) and pectinase (Pec) induced the selective hydrolysis of CamA and CamB, respectively. For the flavonol monoglycoside, two flavonol triglycosides, camelliaside A (CamA) and camelliaside B (CamB) of tea seed extract (TSE) were subjected to hydrolysis in the presence of commercial enzyme complexes (pectinexTM), Mash. Mash induced the hydrolysis of both CamA and CamB, and kaempferol monoglycoside (astragalin, AS) was found to be a main product. In order to prepare AS, enzymatic hydrolysis of TSE using Mash was carried out. Crude NF with 22% purity and AS with 21%
I. INTRODUCTION 1 1.1. Literature review 1 1.1.1. Flavonoid 2 1.1.2. Medicinal properties of flavonoids 6 Antioxidant activity of flavonoids 6 Inhibition of enzymes by flavonoids 6 Dietary antioxidant flavonoids and coronary heart disease 7 Flavonoids with anti-inflammatory activitiy 7 Vascular activity of flavonoids 7 Flavonoids with oestrogenic acitivity 7 Other biological activities of flavonoids 8 1.1.3. Application of plant extracts containing flavonoids 14 1.1.4. Green tea and their use in dermatology 15 1.1.5. Polyphenols in green tea 16 1.1.6. Flavanols in green tea 19 1.1.7. Flavonols in green tea 21 1.1.8. Green tea seed and tea seed extract 23 1.2. Objectives 25 Ⅱ. COLLABORATIVE RESEARCH 27 2.1. Abstract 27 2.2. Introduction 28 2.3. Materials and Methods 31 2.3.1. Materials 31 2.3.2. Analysis of TSE transformation 31 2.3.3. Isolation and quantification of Cam A and B from TSE 32 2.3.4. Enzymatic hydrolysis of TSE 33 2.3.5. Scale-up reaction of NF 33 2.3.6. Purification and identification of NF 33 2.4. Results and Disscussion 35 2.4.1. The separation of CamA and CamB from TSE 35 2.4.2. The amount of CamA and CamB in TSE 35 2.4.3. Enzymatic hydrolysis of TSE 37 2.4.4. Scale-up reaction of NF 46 2.4.5. Purification and identification of NF 48 2.5. Conclusion 53 2.6. Abstract 54 2.7. Introduction 55 2.8. Materials and Methods 59 2.8.1. Materials 59 2.8.2. Analysis of TSE transformation 59 2.8.3. Enzymatic hydrolysis of TSE 60 2.8.4. Scale-up reaction of AS 62 2.8.5. Purification and identification of AS 62 2.9. Results and Discussion 63 2.9.1. Enzymatic hydrolysis of TSE 63 2.9.2. Scale-up reaction of AS 68 2.9.3. Purification and identification of AS 71 2.10. Conclusion 75 Ⅲ. BIOLOGICAL EFFECTS OF PREPARED KAEMPFEROL GLYCOSIDES IN SKIN CELL LINES 77 3.1. Abstra
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College of Engineering(공과대학) > Biotechnology (생명공학) > Theses(생명공학 석박사 학위논문)

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