Thermodynamic properties of the solute transfer from the aqueous acetonitrile mobile phase to the stationary phase monitored by HPLC

Title
Thermodynamic properties of the solute transfer from the aqueous acetonitrile mobile phase to the stationary phase monitored by HPLC
Authors
Cheong, W.J.
Keywords
GAS-CHROMATOGRAPHIC EVIDENCE, LIQUID-CHROMATOGRAPHY, TEMPERATURE-DEPENDENCE
Issue Date
2000-01
Publisher
KOREAN CHEMICAL SOC
Abstract
High-performance liquid chromatography is suitable for getting thermodynamic information about solute-sol vent interactions. We used a squalane impregnated C-18 phase as a presumably bulk-like stationary phase to secure a simple partition mechanism for solute retention in reversed phase liquid chromatographic system. We measured retention data of some selected solutes (benzene, toluene, ethylbenzene, propylbenzene, butylbenzene, phenol, benzylalcohol, phenethylalcohol, benzylacetone, acetophenone, benzonitrile, benzylcyanide) at 25, 30, 35, 40, 45, and 50 degrees C in 30/70, 40/60, 50/50, 60/40 and 70/30 (v/v%) acetonitrile/water eluents. The van't Hoff plots were nicely linear, thus we calculated dependable thermodynamic values such as enthalpies and entropies of solute transfer from the mobile phase to the stationary phase based on more than four retention measurements on different days (or weeks). We found that the cavity formation effect was the major factor in solute distribution between the mobile and stationary phases in the system studied here. Our data were compared with some relevant literature data.
URI
http://dspace.inha.ac.kr/handle/10505/20282
ISSN
0253-2964
Appears in Collections:
College of Natural Science(자연과학대학) > Chemistry (화학) > Journal Papers, Reports(화학 논문, 보고서)
Files in This Item:
posi.pdfDownload

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse