Sphingosine 1-phosphate triggers apoptotic signal for 1316 melanoma cells via ERK and caspase activation

Title
Sphingosine 1-phosphate triggers apoptotic signal for 1316 melanoma cells via ERK and caspase activation
Authors
Shin, J.H.; Choi, G.S
Keywords
sphingosine 1-phosphate, melanoma, apoptosis, caspases
Issue Date
2007-04
Publisher
KOREAN ACAD MEDICAL SCIENCES
Abstract
The bioactive sphingolipid metabolite sphingosine 1-phosphate (S1P), recently was reported to induce apoptosis of some cancer cells and neurons, although it generally known to exert mitogenic and antiapoptotic effects. In this study, we investigated the effects of S1P on the cell growth, melanogenesis, and apoptosis of cultured B16 mouse melanoma cells. In results, S1P was found to induce apoptosis in B16 melanoma cells in a dose- and time-dependent manner, but exerted minimal effects on melanogenesis. Although receptors of sphingosine 1-phosphate (endothelial differentiation gene 1 [Edg]S1P1, Edg5/S1P2, Edg3/S1P3) were expressed in B16 melanoma cells, they were shown not to be associated with S1P-induced apoptosis. In addition, pertussis toxin did not block the apoptotic effects of S1P on B16 melanoma cells. S1P induced caspase-3 activation and the extracellular signal-regulated kinase (ERK) activation. Interestingly, the ERK pathway inhibitor, UO126, reversed the apoptotic effects of S1P on B16 melan ' oma cells. These results suggest that S1P induced apoptosis of B16 melanoma cells via an Edg receptor-independent, pertussis toxin-insensitive pathway, and appears to be associated with the ERK and caspase-3 activation.
URI
http://dspace.inha.ac.kr/handle/10505/1716
ISSN
1011-8934
Appears in Collections:
Medical School/College of Medicine (의학전문대학원/의과대학) > Nursing (간호학) > Journal Papers, Reports(간호학 논문, 보고서)
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