Improved antibiotic susceptibility test of Orientia tsutsugamushi by flow cytometry using monoclonal antibody

Title
Improved antibiotic susceptibility test of Orientia tsutsugamushi by flow cytometry using monoclonal antibody
Authors
Kang, J.S.
Keywords
Orientia tsutsugamushi, doxycycline, antibodies, monoclonal
Issue Date
2007-02
Publisher
KOREAN ACAD MEDICAL SCIENCES
Abstract
Orientia tsutsugamushi causes scrub typhus, which is endemic in many countries in the Asia-Pacific region including Korea. Recent emergence of doxycycline-resistant strains from Thailand has underlined the importance of the susceptibility tests of O.tsutsugamushi to antibiotics. To improve the flow cytometric technique for the susceptibility test, we applied a monoclonal antibody (MAb) in the quantification of O. tsutsugamushi. With using MAb FS15, we determined the doxycycline susceptibility of two strains, Boryong and AFSC-4 strain which is reported to be doxycycline-sensitive and resistant, respectively. The growth of both strains was inhibited to below 10% of the control in the presence of 0.1 mu g/mL or higher concentrations of doxycycline. We suggest that our approach is more quantitative and reproducible than the conventional microscopic methods.
URI
http://dspace.inha.ac.kr/handle/10505/1717
ISSN
1011-8934
Appears in Collections:
Medical School/College of Medicine (의학전문대학원/의과대학) > Medical Science (의학) > Journal Papers, Reports(의학 논문, 보고서)
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